Protective effect of Ulinastatin in rat islet isolation
- VernacularTitle:乌司他丁在大鼠胰岛分离过程中的保护作用
- Author:
Lei YAO
;
Dequan WU
;
Guodong LI
- Publication Type:Journal Article
- Keywords:
Cell separation;
Islet of Langerhans;
Trypsin inhibitors;
Rat
- From:
Chinese Journal of Organ Transplantation
2005;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the protective effect of Ulinastatin in rat islet isolation and advance an improved method in rat pancreas islet isolation.Methods Rat pancreas was perfused with Hanks solution containing collagenase. The rats were divided into 3 group according the Ulinastatin concentrations in collagense: low-concentration Ulinastatin ( 2500 U/ml) group, high-concentration Ulinastatin ( 5000 U/ml) and control group (no Ulinastatin). The isolated pancreases were compared among the groups. Results The shape and number of the isolated pancreases in low-concentration Ulinastatin and high-concentration Ulinastatin groups were superior to those in control group (P
