The expression of SARS-1 by E.coli and its binding with sensitive cells
- VernacularTitle:大肠杆菌表达SARS-S1大片段与SARS敏感细胞的结合
- Author:
Shumei HAO
;
Xuanjun WANG
;
Haibin JI
;
Xiuxia ZHANG
;
Zhiwu WANG
;
Chunping CHI
- Publication Type:Journal Article
- Keywords:
SARS-CoV;
Immunofluorescence;
Receptor;
Ability
- From:
Chinese Journal of Immunology
1999;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the interaction between the SARS-S1 protein and the SARS-sensitive cells.Methods:The SARS-S1 expression in the E.coli was purified and the anti-SARS-S1 was produced.After incubate with the SARS-S1 protein,cells was staining by the mouse-anti-SARS-S1 antibody and the anti-mouse antibody labeled by FITC.The result was determined by FACS.Results:After incubate with the SARS-S1 protein,different cell line have a different reaction to the SARS-S1 antibody.Conclusion:The SARS-S1 protein expressed by E.coli has a specific binding ability to the SARS-sensitive cells.
