F-ATPase subunit uncEBF mRNA expression in Streptococcus mutans clinical isolates
- VernacularTitle:变形链球菌临床株F-ATPase亚基基因uncEBF在mRNA水平的表达
- Author:
Deqin YANG
;
Tianjia LIU
;
Qingguo QI
- Publication Type:Journal Article
- Keywords:
Streptococcus mutans;
F-ATPase;
Semi-quantitative RT-PCR;
Gene Expression
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the gene expression variety of different genotype of F-ATPase subunit uncEBF in Streptococcus mutans (S.mutans) and to evaluate the relationship among uncEBF gene expression levels, genotypes and the acidurance ability of S.mutans. Methods:The relative expression quantity of uncEBF gene against the housekeeping gene recA was determined by the two-step method of semi-quantitative RT-PCR in 18 clinical isolates of S.mutans(7 with genotype A uncEBF and 11 with genotyp B,10 with high acidurance and 8 with low). A gel documentation system and QUANTITY ONES software were used to assay the data. Results:uncEBF mRNA expression level in the isolates with genotype A uncEBF was higher than that in those with genotype B(P
