Relationship between Mutans streptococci transmission from mothers to children and initial adherence properties
- VernacularTitle:变形链球菌母子传播与其对牙面初始粘附关系的研究
- Author:
Song LI
;
Tianjia LIU
;
Deqin YANG
- Publication Type:Journal Article
- Keywords:
Mutans streptococci;
Transmission;
Adherence;
Surface protein
- From:
Journal of Practical Stomatology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the relationship between Mutans streptococci (MS) transmission from mothers to children and its initial adherence properties.Methods:200 MS isolates were genotypied by AP-PCR to demonstrate transmission between 20 pairs of mother and child aged 3~4 years, and to detect the transmitted strains and non-transmitted strains of mothers. Then the adherence of the strains to salivary coated hydroxyapatite beads (SHA) were determined by 3H- thymidine incorperation assay.A restriction fragment length polymorphism (RFLP) study of the different regions(spap-a,spap-pv)of the spap were undertaken by endonuclease haeⅢ and AluⅠrespectively. Results:The transmitted strains showed weaker adherence properties than the non-transmitted strains (P