Study of Effects of Different Levels of Estradiol on Inhibitory of Tamoxifen on Human Mammary Cancer Cells(ER+) in Vitro
- VernacularTitle:雌激素水平对三苯氧胺抑制乳腺癌细胞的体外研究
- Author:
Mingqiang HAN
;
Tao HUANG
;
Yuan TIAN
- Publication Type:Journal Article
- Keywords:
Breast neoplasm Estradiol Tamoxifen
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of different levels estradiol on inhibitory of tamoxifen on human mammary cancer cells(ER+) in vitro.Methods Estrogen receptor(ER)-positive MCF7 human breast cancer cell line was treated by the same level of tamoxifen and different levels of estradiol in vitro.Cell proliferation was evaluated by MTT assay.Results E_2 at concentrations between 1? 10~(-12) mol/L to 1? 10~(-7) mol / L significantly stimulated the growth of MCF-7.TAM(10 ?mol/ L) inhabited the growth of MCF-7 significantly.E_2 at different levels may influence inhibitory of tamoxifen on MCF-7 cell lines.E_2(1?10~(-8) mol/L) makes inhibitory of tamoxifen on MCF-7 cell lines valueless.Conclusion E_2 is the risk factor of breast cancer,and the concentration around breast cancer cells may influence the effects of TAM.
