Effect of Anatoxin-a on the Activity of ATPase in PC12 Cells
- VernacularTitle:类毒素-A对PC12细胞内ATP酶活力的影响
- Author:
Zhihong ZHANG
;
Weidong QU
;
Huigang ZHU
- Publication Type:Journal Article
- Keywords:
Anatoxin-a;
PC12 cells;
ATPase;
Activation;
Desensitization
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of anatoxin-a (ANTX-A) on the activity of ATPase in PC12 cells.Methods PC12 cells were activated by different concentrations of ANTX-A stimulation for an hour or 10-7 mol/L ANTX-A for different time.PC12 cells were desensitized using twice incubation method.The activities of Na+-K+-ATPase and Ca2+-ATPase were determined by colorimetry method.Results 10-9,10-8,10-7 mol/L ANTX-A activated PC12 cells for an hour and 10-7 mol/L ANTX-A activated PC12 cells for 30,60,120 min,the activity of Na+-K+-ATPase in PC12 cells,in all of the cases,showed an reduction tendency,the activity of Ca2+-ATPase decreased significantly in different activation situation compared to the control group(P
