Reactive Oxygen Radicals Generation and Apoptosis in HLF Cells Induced by PM10
- VernacularTitle:PM_(10)诱导人胚肺成纤维细胞自由基产生与其凋亡的作用
- Author:
Xiaohong ZHAO
;
Shengquan MI
;
Yunjie HAO
- Publication Type:Journal Article
- Keywords:
Particle matter;
Radicals;
Apoptosis;
Chitosan oligosaccharides
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The increasing evidences indicate that the inhalable particle matter (PM10) is responsible for respiratory and cardiovascular morbidity and mortality.Generation of superoxide anion,hydroxyl radical and apoptosis in HLF cells induced by PM10 were studied in this paper to present some data for elucidating the mechanism of effects of PM10.Methods The superoxide anion and hydroxyl radical were detected respectively by cytochrome C assay and apoptosis inspected with Annexin V-FITC/PI double staining by flow cytometry.Results PM10 could generate the superoxide anion and hydroxyl radical,with significant dose-dependent manners,and the levels of the superoxide anion as well as hydroxyl radical were decreased by chitosan oligosaccharides (COS),with the significant protection effects (P
