Toxicity of Rotenone to PC12 Cells
- VernacularTitle:鱼藤酮对PC12细胞的毒性作用
- Author:
Mingwei WANG
;
Xiuqiao SUN
;
Ping GU
- Publication Type:Journal Article
- Keywords:
Rotenone;
Apoptosis;
Membrane potential
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the toxic effects of Rotenone on PC12 cells.Methods MTT assay was used to detect the toxic effect of Rotenone.The apoptosis rate and membrane potential of mitochondria were examined by flowctyometry (FCM).Results PC12 cells viability decreased after exposed to Rotenone for 72 hours.Although the apoptosis rate was very low,the G1 phase cells increased and membrane potential decreased as the increase of Rotenone concentration.Mitochondrial membrane potential decreased with a concentration dependent manner.Conclusion Low level and long term exposure of rotenone may induce the membrane potential of mitochondria of PC12 cells decrease,it indicated that rotenone may induce an apoptotic process with the mitochondrial membrane potential decrease in the early stage.
