A Study on Micronuclei and DNA Damage of Bone Marrow Cells of Mice Induced by Holmium Ions of Rare Earths
- VernacularTitle:钬化物诱导小鼠骨髓细胞微核和DNA损伤的研究
- Author:
Chengrun WANG
;
Jun BAO
- Publication Type:Journal Article
- Keywords:
DNA damage;
Holmium;
Micronuclei test;
Single cell gel electrophoresis
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genotoxicity of heavy rare-earth holmium to bone-marrow cells of mice.Methods Kunming mice were randomly divided into six groups and were given holmium-trioxide-HCl by gavage at several doses of 0,10,20,40,80 and 160 mg/kg bw respectively one time a day for two days;other Kunming mice were randomly divided into 3 groups and holmium nitrate was given i.p.at doses of 10,40 and 80 mg/kg bw respectively one time a day for two days.24 hours after the last treatment,the bone-marrow cells of thighbone were collected for micronuclei analysis and single cell gel electrophoresis (SCGE).Results Frequencies of micronuclei were increased by the 2 kinds of holmium solutions at the dosage from 10 to 80 mg/kg bw with a dose-effect relationship,but at the dose of 160 mg/kg bw,it was lower than the negative control.In SCGE,the average tail length increased significantly (P
