Improvement of Suppression Subtractive Hybridization and Applications on Environmental Medicine
- VernacularTitle:抑制消减杂交技术研究进展及其在环境医学上的应用
- Author:
Zhonghai XIAO
;
Xin HONG
;
Zhaoyun YIN
- Publication Type:Journal Article
- Keywords:
Genes;
Suppression subtractive hybridization;
Cloning
- From:
Journal of Environment and Health
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Suppression subtractive hybridization(SSH) is a good and highly effective method for gene cloning,which posses some character,such as convenient operation,high specificity,low background,good replication etc,and it can separate lower specific fragment.This article principally introduced its main technical points,strength and shortcomings and improvement of SSH,and also reviewed its applications in detecting the effects of radiation,heat,hypoxia,coldness and other environmental factors on gene expression.
