Diagnostic implication of human cytomegalovirus immediate early 1 mRNA detection by nucleic acid sequence-based amplification in renal transplant recipients
- VernacularTitle:巨细胞病毒即刻早期mRNA测定在肾移植后巨细胞病毒感染中的诊断价值
- Author:
Yafeng LIU
;
Keli ZHENG
;
Changxi WANG
- Publication Type:Journal Article
- Keywords:
Kidney transplantation;
Cytomegalovirus infections;
Gene amplification
- From:
Chinese Journal of Organ Transplantation
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the significance of immediate early 1 (IE) mRNA detection by nucleic acid sequence-based amplification (NASBA) in the diagnosis of human cytomegalovirus (HCMV) infection after renal transplantation.Methods The expression of IE-mRNA and pp67-(mRNA) was detected by NASBA in 55 patients after renal transplantation, and pp65 antigenemia (assays) were done for all cases.Results Twenty cases were positive for IE-mRNA. Thirteen cases were suffered from CMV disease with symptoms. The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of IE-mRNA by NASBA were 92.3 %, 80.9 %, 60.0 % and 97.1 %, respectively. Time to first IE-mRNA positive detection after transplantation was shortest, compared with pp67 and antigenemia (P
