Indoleamine 2, 3-dioxygenase activity from mesenchymal stem cells suppressing T-lymphocyte responses
- VernacularTitle:骨髓间充质干细胞的吲哚胺2,3-双加氧酶活性抑制T淋巴细胞的增殖
- Author:
Xiaoqiong TANG
;
Zhigang ZHAO
;
Ping ZOU
- Publication Type:Journal Article
- Keywords:
Nematopoietic stem cells;
Tryptophan oxygenase;
T-lymphocytes
- From:
Chinese Journal of Organ Transplantation
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the influence of indoleamine 2, 3-dioxygenase (IDO) (activity) from mesenchymal stem cells (MSCs) on the allogeneic T-lymphocytes responses.Methods MSCs were isolated and cultured from human bone marrow. Selected surface antigens of MSCs were detected by flow cytometry and their morphologic characteristics were determined by microscopy. In MSCs induced by ?-interferon (IFN-?) at different concentrations (0, 20, 50, 100, 200 U/ml), IDO mRNA expression and IDO activity were detected. MSCs Induced by IFN-? at the concentration of 200 U/ml were plated in dishes and then mixed lymphocyte reaction (MLR) cultures were set up. T- (lymphocytes) proliferation was determined by MTT assays and IDO activity was determined by high-performance liquid chromatography (HPLC).Results IFN-? could stimulate the IDO mRNA expression and IDO activity in MSCs in a dose-dependent manner. IDO enzyme activity in MSCs inhibited T-lymphocytes proliferation of MLR cultures.Conclusion MSCs could suppress T-lymphocyte responses via IDO enzyme activity in vitro.
