Establishment of simultaneous liver-kidney transplantation model
- VernacularTitle:大鼠肝、肾联合移植模型的建立
- Author:
Fang YANG
;
Yi JIANG
;
Qiucheng CAI
- Publication Type:Journal Article
- Keywords:
Liver;
Kidney;
Transplantation;
Rats;
Postoperative complications
- From:
Chinese Journal of Organ Transplantation
2005;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore a better method for establishing rat model of simultaneous (liver)-kidney transplantation and the prevention of complications after transplantation.Methods In the donor operation, the liver and the right kidney were flushed with 0-4 ℃ sodium lactate Ringer’s (injection) through aorta, and en bloc resection of organs of right kidney, liver, pancreas, intestines, and spleen were done. Preparation was performed in the preservation solution. During the recipient (operation), all the blood vessels were reconstructed with cuff methods. The bile duct was anastomosed using a simple inside bracket. Operation of connecting ureter attached part of bladder with the bladder was conducted.Results One hundred and ten simultaneous liver-kidney transplantations including 50 (preliminary) experiments were carried out. Eighteen recipient rats died in the preliminary experiments. Five recipient rats died in the formal experiments and all the remaining had survived for more than 30 days. The successful rate of formal transplantations was 91.7 %.Conclusion This kind of rat model of simultaneous liver-kidney transplantation is simple. The time for blood vessel anastomosis and (anhepatic) stage was reduced. Complications such as stenosis were avoided. Preparation before operation, manipulation after operation and management after operation can affect the establishment of the model.
