Study on gene mutations of single S region in immunoglobulin class switch recombination
- VernacularTitle:免疫球蛋白单个转换区在类别转换时基因突变的研究
- Author:
Shenghe TIAN
;
Kazuo KINOSHITA
;
Tasuku HONJO
- Publication Type:Journal Article
- Keywords:
Immunoglobulin;
Class switch;
S region;
Mutation
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the gene rearrangements in immunoglobulin single switch(S) region during class switch recombination.Methods:An artificial substrate containing single S region(FS?-2) driven by tetracycline-responsive promoter was constructed and underwent CSR in a switch-inducible B lymphoma line was stimulated by cytokines,then the S region mutations and its dependent factors were detected by detected PCR and sequencing.Results:Gene rearrangements including deletions,insertions as well as point mutations could occur even in this single S region after cytokines stimulation;Furthermore,when the upstream promoter was suppressed by its inhibitor,CSR frequency was significantly decreased,indicating a transcription dependent S region recombination.Conclusion:Class switch recombination mechanism can exert a similar effect on single S region.
