Apoptosis of pancreatice cancer cells induced by antisense RNA of survivin gene in vitro
- VernacularTitle:survivin反义寡核苷酸在体外诱导胰癌细胞凋亡
- Author:
Pingyuan XIONG
;
Kaiwen GUO
;
Zhaohui TANG
- Publication Type:Journal Article
- Keywords:
Antisense RNA;
survivin;
Pancreatic cancer;
Apoptosis
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effects of survivin antisense RNA on apoptosis in pancreatic cancer cell Panc-1.Methods:survivin antisense RNA was transferred into pancreatic cancer cell Panc-1 by lipofection.The opposite livability on Panc-1 cell line were assayed with MTT test.Expression of survivin detected by RT-PCR.Apoptosis was detected by DNA gel electrophoresis.Results:survivin antisense RNA obviously inhibited the Panc-1 cell growth(P
