Expression of kringle 1-3 domain fragment of human plasmihogen in E.coli and the anti-tumor activity of its product
- VernacularTitle:人纤溶酶原K1-3基因的表达及抗肿瘤作用研究
- Author:
Xingyan LU
;
Tianyuan ZHANG
;
Jinxian LUO
- Publication Type:Journal Article
- Keywords:
Plasminogen K1-3 domain fragment;
Gene cloning and expression;
Anti-angiogenesis;
Anti-tumor
- From:
Chinese Journal of Immunology
2000;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of kringle 1-3 domain fragment of human plasmihogen in E.coli and the anti-tumor activity of its product.Methods:The K1-3 domain fragment was cloned in expression vector pBV220,the resulted recombinant plasmid pBV-K13 was transformed into E.coli DH5? and its product was purified and assayed its bioactivity.Results:K1-3 domain fragment was expressed in(E.coli) DH5?.The results showed the expressed product covered 20% of the total bacterial protein on SDS-PAGE and the Western blot analysis showed that the product had immunological specificity with the antiserum of human plasminogen and inhibits the growth of chorioallantoic membrane(CAM) angiogenesis and mouse B16 melanoma.Conclusion:Human plasminogen K1-3 domain fragment was expressed in E.coli;the expressed product has anti-angiogenesis and anti-tumor activity.
