Effect of nitric oxide on the prostatic proliferation/apoptosis in rats
- VernacularTitle:一氧化氮对大鼠前列腺增殖/凋亡的影响
- Author:
Cheng SHEN
;
Jing PENG
;
Yinglu GUO
;
Jie JIN
- Publication Type:Journal Article
- Keywords:
Prostate;
Proliferation;
Apoptosis;
Nitric oxide synthase;
Nitric oxide
- From:
Chinese Journal of Urology
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of nitric oxide(NO)on the prostatic proliferation/apoptosis in rat models. Methods Twenty adult male rats were randomly divided into 3 groups.Group 1 were normal controls (n=6);group 2 (n=7) and group 3 (n=7) were treated with subcutaneous injection of nitric oxide synthase (NOS) inhibitor-L-NG-Nitroarginine methyl ester (L-NAME) 50mg/kg and 100mg/kg, bid, respectively.The weight of the prostate was measured after 2 weeks and morphological changes were examined with light microscope.Detection of ki-67 and TUNEL in prostatic epithelial cells were undertaken so as to assess the proliferation and apoptosis index.The protein and mRNA expression of NOS were analyzed by means of immunohistochemistry and RT-PCR. Results The prostate weight of 3 groups was not significantly different.Compared with group 1, remarkable atrophy of prostatic epithelial cells,marked decrease in proliferation rate of epithelial and interstitial cells, and significant increase in apoptosis rate of epithelial cells were noted in group 2 and group 3 (P
