Effects of neferine on expression of glutathione S-transferase-pi in K562/A02 cells in vitro
- VernacularTitle:甲基莲心碱抑制K562/A02细胞GST-?的表达
- Author:
Xibin XIAO
;
Zhaoxia XIE
;
Qun QIN
- Publication Type:Journal Article
- Keywords:
leukemia;
drug resistance,multiple;
neferine;
glutathione S-transferase-?
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of neferine on the expression of glutathione S-transferase-pi in vitro and to explore the multi-drug resistance reversing mechanism of neferine.Methods 50% inhibition concentration(IC_(50)) of ADM on K562/A02 was determined by MTT method.The transcription of GST-? gene was detected by semi-quantitative RT-PCR and the expression level of GST-? was determined by Western blot after neferine treatment.Results Neferine remarkably enhanced chemosensitivity to ADM of K562/A02 cells.After neferine treatment in day 1,day 3 and day 5,the relative efficiency of K562/A02 to ADM was 9.6%,41.4% and 10.7%,respectively.Semi-quantitative RT-PCR showed that mRNA transcription of GST-? gene was significantly reduced(P
