Preliminary application of rep-PCR in epidemiological research of extended spetrum ?-lactamase(ESBL)+ Escherichia coli
- VernacularTitle:rep-PCR法在检测产超广谱?内酰胺酶大肠艾希氏菌院内流行中的应用
- Author:
Lieting MA
;
Duo YANG
;
Yawen WANG
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
polymerase chain reaction;
epidemiology
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish better reaction factors for rep-PCR and to investigate if the extended spectrum ?-lactamase(ESBL)+ Escherichia coli isolated from different patients in the respiratory ward have the same origin by using rep-PCR.Methods The ESBL confirmation was taken by double disc confirmatory test.The susceptibility testing was performed with K-B test.By applying the widespread enterobacter repetitive intergenic consensus as a primer,the stains were typed by rep-PCR following electrophoresis in agarose gel.Results The analysis of the PCR productions indicated that it would create useful DNA ban-prints and all these 22 ESBL(+)Escherichia coli strains were of three origins.Conclusion The method of rep-PCR is practical for epidemiological research in nosocomial infection.
