Immunological effects of 1,25-dihydroxyvitamin D_3 on experimental allergic encephalomyelitis in Lewis rats
- VernacularTitle:实验性自身免疫性脑脊髓炎大鼠中1,25二羟基维生素D_3的免疫调节作用
- Author:
Feng ZHANG
;
Yuhong FU
;
Weizhi WANG
;
Baofeng YANG
- Publication Type:Journal Article
- Keywords:
Encephalomyelitis,experimental autoimmune;
Calcitriol
- From:
Chinese Journal of Neurology
2005;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on experimental allergic encephalomyelitis (EAE) and its immune-modulating mechanisms. Methods 1,25-(OH)2D3 were given to Lewis rats immunized with myelin basic protein (MBP) in complete Freud’s adjuvant (CFA) respectively on the same day of immunization (the prevented group) and the presenting day of symptoms (the treated group). Then clinical symptoms were scored until the 13th day after immunization, when rats were sacrificed. Mononuclear cells (MNC) in draining lymph nodes were collected for the following studies: (1)Total number of MNC in the lymph nodes were counted. (2) Numbers of CD~+4CD~+ 25 cells and CD~+ 86 cells were determinated by flow cytometry. (3) The concentrations of interferon(IFN)-? and interleukin(IL)-4 in cell culture supernatant were measured by enzyme-linked immuno sorbent assay (ELISA). Results 1,25-(OH)2D3 could delay the peak EAE severity in the prevented group while it could relieve the progression of EAE and lower the peak score in the treated group (3.3?0.6 vs 4.0?0.3, P
