Dendritic cells modified with interleukin 23 acquire antigen from apoptotic pancreatic carcinoma cell and induce CTLs immune response
- VernacularTitle:凋亡癌细胞抗原致敏IL-23基因转染的树突细胞抗肿瘤免疫反应的研究
- Author:
Guang TAN
;
Zhongyu WANG
;
Shuo YIN
- Publication Type:Journal Article
- Keywords:
Dendritic cell;
IL-23 gene;
Pancreatic carcinoma;
Immune response
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the immune response to murine pancreatic carcinoma by bone marrow-derived dendritic cells(BM-DCs) modified with interleukin-23 after acquiring antigen from apoptotic pancreatic carcinoma cell.Methods:The murine IL-23 cDNA was sub-cloned into dual-expression vector.DCs were pulsed with apoptotic tumor cell antigen after transduced with interleukin-23 gene.The immune preventative and immunotherapeutic effects of DC vaccines on mice with pancreatic cancer were assessed.Results:IL-23 protein could apparently increase the antigen presenting ability of DC.After the vaccination of DC vaccines.IFN-? production in treatment group were significantly more than that of control group(P
