The influence of the total glucosides of radix paeoniae rubra on the expression of Bcl-2,c-myc gene and apoptosis mechanisms in tumor cell
- VernacularTitle:赤芍总甙对Bcl-2、c-myc基因表达影响及诱导细胞凋亡机制研究
- Author:
Huiyu XU
;
Guangyin WANG
;
Zhiwei CHEN
- Publication Type:Journal Article
- Keywords:
Total glucosides of radix paeoniae(TGC);
Bcl-2;
c-myc;
Apoptosis corpuscle
- From:
Chinese Journal of Immunology
2001;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study TGC on tumor apopotosis and to apply the experimenting basis of TGC's developing and utilizing.Methods:The flow cytometer(FCM) was used for determination apoptosis.The protein expression of Bcl-2 and the mRNA expression of c-myc were evaluated by immunohistochemistry and in situ hybridization.Apoptosis small body were observed by EU.Results:The TGC group appear the Ap apex,the number of cell of G0-G1 period reduce obviously,the cells of S period increase obviously.Bcl-2 and the c-myc mRNA expression levels were decreased in TGC group.The structure of inner membrane was intacted with EU.Chromatin gathered side of cell,the nucleus turns into the nucleus band and nucleus project,there were many cells of the apoptosis and meniscus or the garland shape(the apoptosis corpuscle formatiol) in TGC group.Conclusion:The total glucosides of radix paeoniae induces the tumor cell apoptosising,its mechanism maybe closely related with adjusting expression levels of the Bcl-2 and the c-myc mRNA.
