Effect of intraspinal grafting of adenovirus-mediated brain derived neurotrophic factor ex vivo transgene myoblasts cells and methylprednisolone on expression of caspase-3 after spinal cord injury
- VernacularTitle:脑源性神经营养因子转基因细胞移植和甲基强的松龙对损伤脊髓caspase-3表达的影响
- Author:
Qiang ZHANG
;
Dewei ZOU
;
Yong HAI
;
Huasong MA
;
Yongjun WANG
- Publication Type:Journal Article
- Keywords:
Spinal cord injuries;
Transgenes;
Brain derived neurotrophic factor;
Caspases;
Methylprednisolone
- From:
Chinese Journal of Trauma
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of intraspinal grafting of brain derived neurotrophic factor (BDNF) ex vivo transgene myoblasts cells and methylprednisolone on caspase-3 expression after spinal cord injury (SCI). Methods A total of 120 experimental rats were divided into Group A (spinal cord contusion injury group), Group B (grafting of BDNF ex vivo transgene myoblasts cells group), Group C (methylprednisolone intravenous injection group) and Group D (grafting of BDNF ex vivo transgene myoblasts cells and methylprednisolone intravenous injection group). At days 1, 3, 7, 14 and 28 respectively after SCI, the expression of caspase-3 was measured immunohistochemically for quantitative analysis via a computer image analysis system. The motion functional recovery of the rats was observed by praxiologic and electrophysiologic examination. Results Positive expression cells of caspase-3 were found in all groups, with number from the highest to the lowest in order of Group A, Group B, Group C and Group D (P
