Protective effects of tempol on ultraviolet-B exposed human foreskin fibroblasts
- VernacularTitle:氮氧化物对中波紫外线照射下包皮成纤维细胞的保护作用
- Author:
Shuxian YAN
;
Yu XU
;
Yue HU
;
Kanghuang LIAO
- Publication Type:Journal Article
- Keywords:
Skin aging;
Fibroblasts;
Ultraviolet rays;
Cyclic N-oxides
- From:
Chinese Journal of Geriatrics
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the effects of tempol(a nitroxide), in the exposure of ultraviolet-B (UVB), on cell proliferation, superoxide enzyme (SOD) activity, lipid peroxidation, and expression of matrix metalloproteinases (MMP)-1,MMP-3 in human foreskin fibroblasts in vitro. Methods Fibroblasts were irradiated by a single exposure of 36 seconds to 40 mJ/cm 2 UVB and at the same time incubated with, or without, tempol and detected twenty-four hours later. SOD activity and lipid peroxidation,as shown by accumulation malondialdehyde (MDA) were detected by biochemical assay. Expression of MMP-1 and MMP-3 (mRNA level) were examined by Semi-quantitative RT-PCR. Results 40 mJ/cm 2 UVB significantly inhibited cell proliferation rate to (84?8)% (P
