A new simplified method of platelet-rich plasma preparation
- VernacularTitle:传统二次离心法和改良法制备富血小板血浆的比较研究
- Author:
Song YANG
;
Gong PING
;
Hou RUI
- Publication Type:Journal Article
- Keywords:
Platelet-rich plasma;
Transforming growth factors-?1;
Enzyme-linked immunoassay
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To improve the method for isolating platelet-rich plasma (PRP). Methods:Whole blood was collected from 8 healthy donors and then PRP was separated by both the tube method and the syringe method respectively. Samples were activated to get serum rich-in growth factors (SRGF).Platelets in the SRGF were counted and the level of TGF-?1 was assayed by enzyme-linked immunosorbent assay (ELISA).Results:The end product of syringe method has both a higher platelet count in PRP (P=0.003) and a higher level of TGF-?1 in SRGF(P=0.041) than that of tube method.Conclusion:The syringe method is an effective method in preparation of PRP.
