Preparation of bifunctional anti-human lingual cancer/anti-VEGF antibody
- VernacularTitle:抗人舌癌/抗VEGF双功能抗体的制备及应用研究
- Author:
Yingxin CHEN
;
Wenxin GAO
;
Wang LEI
- Publication Type:Journal Article
- Keywords:
Tongue neoplasms;
Vascular endothelial growth factor;
Bispecific antibody
- From:
Journal of Practical Stomatology
2000;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop bifunctional anti-human lingual cancer/anti-VEGF antibody.Methods:Antigen of lingual cancer was prepared from Tca 8113 cells and that of VEGF from endothelial cells of umbilical cord vein.The hybridoma cells of anti-human ligual cancer/anti-VEGF were prepared by the technique of secondary hybridoma.The antibody was identified by ELISA. Results:The specific conjugation rate between bifunctional antibody and lingual cancer tissue was 85.29%, and that between the antibody and vascular endothelial cells was 82.35%.Conclusion:The bifunctional antibody of anti-human lingual cancer/anti-VEGF has good targeting potential.
