Influnence of ET-1 on [Ca~(2+)]i in cultured bovine trabecular meshwork cells in vitro
- VernacularTitle:内皮素-1对体外培养的牛眼小梁细胞内钙离子浓度的影响
- Author:
Huiqin LU
- Publication Type:Journal Article
- Keywords:
trabecular meshwork cells(TMC);
ET-1;
[Ca~(2+)]i;
LSCM
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2004;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To find out the method of ET-1 in regulating trabecular meshwork cell(TMC) contraction and to explore new effective drug treating glaucoma with little side-effects.Methods TMCs were obtained through the cultured tissue,and then loaded with Fluo-3/AM,the value of [Ca~(2+)]i was obtained by dynamically scanning the changes of intracellular fluorescent intensity after the application of different concentration of ET-1.Results The application of ET-1(10~(-9),10~(-8)and 10~(-7)mol/L),led to an increase in([Ca~(2+)]i.) Especially,the application of 10~(-8) mol/L ET-1 led to a significant increase in [Ca~(2+)]i: from(327.50)?(24.57) to 373.60?40.18(n=8,P
