Protective Effects of Epidermal Growth Factor on Pancreas of Rats with Acute Pancreatitis
- VernacularTitle:表皮生长因子对急性胰腺炎大鼠胰腺的保护作用
- Author:
Dongli CHEN
;
Weizhong WANG
;
Xiaonan LIU
- Publication Type:Journal Article
- Keywords:
Epidermal growth factor Acute pancreatitis Ascites Malondialdehyde Apoptosis
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effects of epidermal growth factor (EGF) on pancreas of rats with acute pancreatitis(AP). Methods Seventy-two male Sprague-Dawley rats were randomly divided into 3 groups: Control group, AP group and AP-EGF group. Subcutaneously injection of EGF (0.1 ?g/g) were given to animals in the AP-EGF group after the establishment of the model of AP. The other two groups of animals received the same volume of saline. At 6 h, 12 h and 24 h after induction of AP, 8 animals in each group were sacrificed respectively, 4 ml of blood sample was withdrawn from heart,2 ml for the analysis of amylase activity and 2 ml for MDA content in serum. Ascites was sucked with dry gauzes and was weighed thereafter. Changes of pancreas morphology were evaluated at every time point. The same part of pancreas was removed for measurement of MDA content, apoptotic index (AI) and histologic changes. Results Histologic injury of the animals in the AP-EGF group was milder than that in the AP group. Ascites weight in the AP-EGF group decreased significantly compared with that in the AP group at 12 h and 24 h 〔(4.53?1.29) g vs (6.58?1.47) g, (7.64?1.85) g vs (11.96?2.13) g,P
