Human Interleukin-13 Affects Expression of Adhesive Molecules on Bovine Aortic Endothelial Cells
- VernacularTitle:人白介素-13对牛主动脉内皮细胞表达黏附分子的影响
- Author:
Gang CHEN
;
Zhiren FU
;
Lianghua WANG
;
Binghua JIAO
- Publication Type:Journal Article
- Keywords:
Human interlukin-13 Bovine aortic endothelial cell Intercellular adhesion molecule-1 E-selectin
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of human interlukin-13 (hIL-13) on the expression of E- selectin and intercellular adhesion molecule-1(ICAM-1) on bovine aortic endothelial cells(BAECs) stimulated by tumor necrosis factor alpha(TNF-?), and to provide experimental basis for hIL-13 inducing immunity endure and relieving the repulsion reaction of xenograft. Methods BAECs were co-cultured with different concentrations of hIL-13 for 2 h and followed by co-cultured with 4 ng/ml TNF-? for 6 h or 18 h. The expressions of E-selectin and ICAM-1 on BAECs were detected by Cell-ELISA. The effect of hIL-13 on activity of BAECs was detected by MTT colorimetry.Results BAECs pretreateded with hIL-13 could inhibit the expression of E-selectin and ICAM-1 induced by TNF-?, and showed a does-dependent manner from 5 ng/ml to 20 ng/ml of hIL-13 (P
