Effect of Hypoxia on Expression of VEGF in HepG2 Cells
- VernacularTitle:缺氧对肝癌细胞系HepG2表达VEGF的影响
- Author:
Xingrui LI
;
Xiaofeng LIAO
;
Jilin YI
- Publication Type:Journal Article
- Keywords:
Hepatocellular carcinoma Vascular endothelial growth factor Transcatheterized hepatic arterial chemoembolization
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of vascular endothelial growth factor (VEGF) expression in HepG2 cells under hypoxia. Methods HepG2 cells were cultured under hypoxia(hypoxia group) and normal condition (control group). VEGF expression of HepG2 cells was examined by immunohistochemical staining. The growth of HepG2 cells was examined by MTT colorimetry and cell count. VEGF level in the culture medium was measured by ELISA.Results After 48 h and 72 h of culture, the growth rate of HepG2 cells in hypoxia group was lower than that in control group (P
