Study of Mechanism of Apoptotic Signal Transduction in Human Hepatic Carcinoma Cell Lines Induced by TGF-?1
- VernacularTitle:TGF-?1诱导人肝癌细胞系凋亡信号转导机理的研究
- Author:
Chunlei WANG
;
Zhiqiang HUANG
;
Yuanlian WAN
;
Behrns KEVIN
- Publication Type:Journal Article
- Keywords:
Transforming growth factor-?1 Apoptosis Hepatoma cell line Signal transduction pathway
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the apoptosis induced by TGF-?1 in human hepatic carcinoma cell lines and its relationship with p53 gene and Smad. Methods Three human hepatic carcinoma cell lines which involving in various status of the p53 gene were used in this study. TGF-?1-induced apoptosis in hepatic carcinoma cell lines was measured by the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. To study the mechanism of TGF-?1-induced apoptosis, these cell lines were transfected with a TGF-?1-inducible luciferase reporter plasmid containing Smad 4 binding elements (SBE) and luciferase gene using Lipofectamine 2000, then treated with TGF-?1, relative luciferase activity was assayed. Results Of three cell lines studied with TUNEL assay, TGF-?1 induced apoptosis was observed in HepG2 cells (wild type p53). Huh-7 (mutant p53) and Hep3B (deleted p53) cell lines showed less apoptosis. Luciferase activity assay indicated that the response to TGF-?1 induction in HepG2 cells was increased dramatically but was not significant in Huh-7 and Hep3B cell lines. Conclusion HepG2 cells seem to be highly susceptible to TGF-?1-induced apoptosis compared with Hep3B and Huh-7 cell lines. Smad 4 is a central mediator of the TGF-?1 signal transduction pathway.
