Effect of Abstract of Particulates Sampled From the Air of a Railway Transport Station of Hazard Chemicals on Rat TypeⅡ Pneumocytes and the Antagonism of Antioxidants
- VernacularTitle:铁路危险品货运站空气颗粒提取物对大鼠肺细胞的损伤及抗氧化剂的干预作用
- Author:
Xujun ZHANG
;
Yuchuan LU
- Publication Type:Journal Article
- Keywords:
Air pollution;
Particulate;
Railway transport station of hazard chemicals;
Antioxidant;
Rat pneumocytes
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To know the effect ofof the particulates sampled from the air of the railway transport station of hazard chemicals on rat typeⅡpneumocytes and the antagonism of antioxidants. Methods The rat typeⅡpneumocytes cultured by routine method were pre-treated with antioxidants N-acetylcysteine (NAC), Vitamin C and Vitamin E, then were exposed to the abstract of particulates. The damages of rat typeⅡpneumocytes and DNA cross-links were detected with MTT assay and ethidium bromide fluorescence assay. Results The abstract of particulates caused obvious damages and DNA cross-links in the rat typeⅡpneumocytes and the significant dose-effect relationship was seen. NAC, Vitamin C and Vitamin E reduced significantly the damages and DNA cross-links. Conclusion Abstract of the particulates sampled from the air of the railway transport station of hazard chemicals may induce the damages and DNA cross-links in rat typeⅡpneumocytes and these adverse effects can be antagonized by antioxidants such as NAC, Vitamin C and Vitamin E.
