The Effect of Gadd Genes Expression in Surveillance of DNA Damage Induced by Nitrogen Mustard
- VernacularTitle:生长抑制和DNA损伤基因表达对氮芥所致DNA损伤的监视作用
- Author:
Shuidong FENG
;
Feng CHEN
;
Hongyan LING
- Publication Type:Journal Article
- Keywords:
DNA damage;
Nitrogen mustard;
Alkylating agents;
Gadd genes
- From:
Journal of Environment and Health
1992;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of Gadd genes expression in surveillance of DNA damage. Methods The human liver tumor cells were treated with alkylating agent(nitrogen mustard) and total RNA was extracted, the expression levels of Gadd genes were determined by RT-PCR. Results Treated with 1 ng/?l nitrogen mustard for 3 h, 24 h, 48 h respectively and with 1 ng/?l, 5 ng/?l nitrogen mustard for 24 h respectively, the expression levels of Gadd genes increased at first (P0.05). Conclusion Gadd genes expression can be used as the indexes in the surveillance of DNA damage induced by nitrogen mustard.
