Effects of Methylprednisolone on TGF-beta, Apoptosis and Renal Scarring in Experimental Acute Pyelonephritic Weaning Rats.
- Author:
Soo Ja WHANG
1
;
Soon Hee SUNG
;
Seung Joo LEE
Author Information
1. Department of Pediatrics, Ewha Womans University, College of Medicine, Korea. sjoolee@mm.ewha.ac.kr
- Publication Type:Original Article
- Keywords:
methylprednisolone;
TGF-beta;
apoptosis;
renal scar;
pyelonephritic weaning rat
- MeSH:
Animals;
Anti-Bacterial Agents;
Apoptosis*;
Atrophy;
Catheters;
Ceftriaxone;
Cell Proliferation;
Cicatrix*;
Clinical Protocols;
Escherichia coli;
Fibrosis;
Inflammation;
Kidney;
Methylprednisolone*;
Mortality;
Pyelonephritis;
Rats*;
Rats, Sprague-Dawley;
Silicones;
Sodium Chloride;
Transforming Growth Factor beta*;
Transforming Growth Factor beta1;
Urinary Bladder;
Weaning*
- From:Journal of the Korean Society of Pediatric Nephrology
2002;6(1):75-84
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Acute pyelonephritis of growing kidneys may result in renal scarring. TGF-beta, inflammatory cytokine, has been suggested to play an important role in promoting renal scarring through apoptosis, suppression of cellular proliferation and fibrosis. We observed the effects of a potent anti-inflammatory agent, methylprednisolone on apoptosis and renal scarring in experimentally induced acute pyelonephritic weaning rats. MATERIALS AND METHODS: To induce ascending pyelonephritis a saline solution containing Escherichia coli type ATCC No. 25922, pili-form (107 bacteria/mL) was infused into the bladder through the 16-guage silicone cannula for 48 hours to 102 three-week-old Sprague-Dawley rats (50-60g). Experimental groups were divided into three groups according to the treatment protocols, group I (ceftriaxone only, n=31), group II (methylprednisolone+ceftriaxone n=28), control group (n=43) was not treated. Histopathologic scores of inflammatory changes, fibrosis and tubular atrophy, the apoptosis index and TGF-beta1 expression score were observed at post-infection 1 and 3 week. Datas were analysed using ANOVA test and P value below 0.05 was interpreted as significant. RESULTS: The mortality rate (21.4%) of group II was not different to the control group (41.9%) and group I (32.3%). The inflammatory score of group II (0.8+/-0.87) at week 1 was significantly lower than those of the control group (2.3+/-0.87) and Group I (1.7+/-0.79) (P<0.05). Apoptosis index of group II (2.9+/-2.15) at week 1 was significantly lower than (10.0+/-0.95) and group 1 (8.3+/-2.53) (P<0.05). TGF-beta1 expression score of group II (0.8+/-0.72) at week 1 was significantly lower than those of the control group (1.9+/-0.68) and group I (1.8+/-0.60) (P<0.05). The fibrosis score of group II (1.1+/-0.10) at week 3 was significantly lower than that of the group I (1.8+/-0.83) (P<0.05). CONCLUSION: Combined treatment with methylprednisolone and ceftriaxone reduced inflammation, fibrosis, apoptosis and TGF-beta expression in acute pyelonephritic weaning rats, compared to ceftriaxone alone. Anti-inflammatory agent supplemented to antibiotics could prevent renal scarring more effectively.
