Protective effect of heme oxygenase-1 for xeno-heart transplantation
- VernacularTitle:血红素氧合酶-1对移植的异种心脏的保护作用
- Author:
Shengchun LIU
;
Zhenxiang YAO
;
Hua TANG
- Publication Type:Journal Article
- Keywords:
Oxygenases;
Transplantation,Heterologous;
Heart transplantation;
Graft survival
- From:
Chinese Journal of Organ Transplantation
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of heme oxygenase-1 for xeno-heart transplantation and the possible mechanism.Methods Cobalt protoporphyrin (CoPP), HO-1 inducer, and zinc protoporphyrin (ZnPP), HO-1 inhibitor, were used to intervene donors and recipients on the model of NIH-Wistar cardiac transplants respectively and simultaneously some of recipients were treated with immunesuppressor cyclosporine A (CsA), and control group and CsA treated group were set up respectively. The expression of HO-1 protein, HO-1 mRNA, caspase-3 and STAT-3 in transplanted heart tissue was detected by immunohistochemistry, RT-PCR and Western Blot. At the same time, HO-1 enzymatic activity was examined in heart tissue, and the cardiac cell apoptosis was examined by means of TUNEL. The differences among various factors were compared.Results The survival time of cardiac transplants in CoPP group was longer than that in control and ZnPP groups (P
