Primary identification of the expression of ?Gal epitope in porcine embryonic fibroblast
- VernacularTitle:猪胚胎成纤维细胞?Gal表达的鉴定
- Author:
Kun WANG
;
Guohua ZHUANG
;
Tonglin ZHANG
- Publication Type:Journal Article
- Keywords:
Swine;
Embryo;
Fibroblasts;
Galactosyltransferases
- From:
Chinese Journal of Organ Transplantation
2005;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the synthesis of ?1,3galactosyltransferase gene in porcine embryonic fibroblast.Methods The transcription and translation of ?1,3galactosyltransferase gene were identified in porcine embryonic fibroblast by RT-PCR and Western blot.Results It was identified that there was the expression of ?1,3 galactosyltransferase gene in the cultured porcine embryonic fibroblasts.Conclusion ?1,3 galactosyltransferase gene can be synthesized in porcine embryonic fibroblast. RT-PCR and Western blot can be applied to identify the expression of ?1,3 galactosyltransferase gene in the porcine embryonic fibroblast.
