A preliminary study on the effects of high glucose and the leucocyte of diabetic patients on endothelial cells
- VernacularTitle:高糖及糖尿病患者白细胞对内皮细胞明胶酶的影响
- Author:
Lixin GUO
;
Mingxiao SUN
;
Dongxiao LI
;
Yingsheng ZHOU
;
Xiaoxia WANG
;
Hui LI
- Publication Type:Journal Article
- Keywords:
Gelatinase B;
Endothelial cells;
Glucose solution, hypertonic
- From:
Chinese Journal of Geriatrics
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of high glucose and the leucocyte of diabetic patients on endothelial cells. Methods The leucocyte adhesion percentage to endothelial cells and the (MMP-9) expression of co-culturing endothelial cells and leucocytes were determined under different levels of glucose and at different time. Results (1) The level of MMP-9 was much higher under high glucose culture, and it increased as culture time prolonged. (2) The percentage of leucocyte adhesion to endothelial cells under high level of glucose was increased, and it was further enhanced when (co-culture) with leucocytes of diabetic patients . (3) The level of MMP-9 in supernatant of endothelial cells and leucocytes was increased significantly after culture with leucocytes of diabetic patients. It showed leucocytes were toxic to endothelial cells. Conclusions High glucose increases the level of MMP-9 of endothelial cells and percentage of the leucocyte adhesion to endothelial cells.Leucocytes of diabetic patients are toxic for endothelial cells.
