Effect of radix paeoniae rubra on expression of heme oxygenase and inducible nitric oxide synthesis in lipopolysaccharide-induced acute lung injury in rats
- VernacularTitle:赤芍对内毒素性急性肺损伤大鼠肺诱导型血红素氧化酶和诱导型一氧化氮合酶表达的影响
- Author:
Zhongyuan XIA
;
Chang CHEN
;
Xiaoyuan WANG
- Publication Type:Journal Article
- Keywords:
Respiratory distress syndrome, acute;
Lipopolysaccharide;
Hemeoxygenase;
Nitric oxide synthase;
Radix paeoniae rubra
- From:
Chinese Journal of Trauma
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of radix paeoniae rubra (RPR) on expression of heme oxygenase (HO-1) and inducible nitric oxide synthase (iNOS) in lipopolysaccharide (LPS)-induced acute lung injury (ALI) and explore its protective mechanism. Methods Forty Wistar rats were randomly and equally divided into five groups, ie, control group, LPS group, RPR treatment group, RPR prevention group and Hemin group. Arterial blood was drawn for blood gas analysis. Models of endotoxin-induced ALI were used to observe the protein content, the ratio of neutrophiles in bronchoalveolar lavage fluid (BALF), the malondialdehyde (MDA) content in lung and the activities of serum NO. Expression of HO-1 and iNOS in rat lung tissue was detected by immunohistochemistry and morphometry computer image analysis. The histological change of lung were observed under light microscope. Results Compared with control group, expression of HO-1 and iNOS was markedly increased (P
