The effect of TGF-?_2 on the expression and activity of MMP_2 in cultured bovine trabecular cells
- VernacularTitle:TGF-?_2对体外培养的牛眼小梁细胞MMP_2表达和活性的影响
- Author:
Siwei LIU
;
Ying XIAO
;
Junze MA
- Publication Type:Journal Article
- Keywords:
bovine trabecular cells;
transforming growth factor;
matrix metalloproteinase;
primary open angle glaucoma
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the effect of transforming growth factor-?_(2)(TGF-?_(2)) on the expression and activity of matrix metalloproteinase-2(MMP_(2)) in cultured bovine trabecular cells and to investigate the role of TGF-?_(2) in the pathogenesis of primary open angle glaucoma(POAG). Methods TM cells were incubated in DMEM containing 1.0?g/L TGF-?_(2) or 1.0?g/L TGF-?_(2) with 100mg/L murine monocolonal anti-human PAI-1 IgG or DMEM without any experimental reagent for 24, 36, 48h, respectively. The expression of MMP_(2) or plasminogen activator inhibitor-1(PAI-1) were assayed by Western blot. Results TGF-?_(2 )could enhance the expression of pre-form MMP_(2) and PAI-1. PAI-1 neutralizing antibody could promote transformation of the pre-form MMP_(2) to it's active form. Conclusion TGF-?_(2) can cause the accumulation of ECM in trabecular meshwork by inhibiting the activation process of pre-form MMP_(2), and thus cause the increase of aqueous humor outflow resistance, which contributes to the pathogenesis of POAG.
