Correlation between K-ras gene mutation and non-small cell lung cancer
- VernacularTitle:K-ras基因点突变在非小细胞肺癌中的意义
- Author:
Jianjie ZHANG
- Publication Type:Journal Article
- Keywords:
NSCLC;
PCR;
K-ras mutation
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the clinical significance of K-ras mutation in non-small cell lung cancer. Methods A K-ras codon 12 point mutation was detected by restriction fragment length polymorphisms combined with polymerase chain reaction in 41 tissues from non-small cell lung cancer (NSCLC), 21 normal tissues from nearby tumor and 21 lymph nodes from nearby bronchi. Results K-ras mutation was detected in 10 of 41 NSCLC((24.39%)). There was no relationship between K-ras mutation and the age, sex, differentiation of tumor, tumor volume,histologic type and tumor stage. K-ras mutation was more common in smoking group than in non-smoking group, but the statistical comparison was not significant. There was a strong link between cigarette smoking and K-ras point mutation in lung adenocarcinoma, but there was no relation between cigarette smoking amount and K-ras mutation. K-ras mutation was found in 4 of 21 metastatic lymph notes. The mortality in positive K-ras mutation was significantly higher than that in negative K-ras mutation(P
