The role of transcription factor NF- ?B in vascular endothelial cell impairment induced by high glucose,TNF-? and IL-1?
- VernacularTitle:NF-?B在高浓度葡萄糖、TNF-?、IL-1?介导的血管内皮细胞损害中的作用
- Author:
Yujun XIAO
;
Ailong HUANG
;
Chunlin LI
;
Huacong DENG
;
Mingque XIANG
;
Ni TANG
- Publication Type:Journal Article
- Keywords:
NF-?B;
Glucose;
Cytokines;
Vascular endothelial cells
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of NF-?B in high glucose (HG) and cytokines (TNF-? and IL-1?)-induced impairment of ECV-304 cells (vascular endothelial cell line). Methods Recombinant adenovirus containing NF-?B supper-repressor I?B?M with mutant I?B? was constructed. Western blot, electrophoretic mobility shift assay (EMSA) and thiazolyl blue viability assay were applied in this study. Results TNF-?-induced I?B? degradation and NF-?B activation (P
