Inhibitive effect of tea polyphenols on the growth of human thyroid papillary carcinoma cells
- VernacularTitle:茶多酚抑制人甲状腺乳头状癌细胞生长
- Author:
Yanping XIA
;
Maohua YU
- Publication Type:Journal Article
- Keywords:
Tea polyphenols;
CGTH W-3 cells;
Apoptosis
- From:
Chinese Journal of Endocrinology and Metabolism
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of tea polyphenols on proliferation, invasion and apoptosis of human thyroid papillary carcinoma cells (CGTH W-3) in vitro. Methods The effects of various concentrations of tea polyphenols on proliferation, invasion and apoptosis of CGTH W-3 cells were observed by MTT assay, plate scarification assay and flow cytometer, respectively. Results Tea polyphenols(50-400 mg/L) inhibited the proliferation of CGTH W-3 cells with dose-and time-dependent manners. The IC_50 of tea polyphenols to CGTH W-3 cells was 240 mg/L (1st day), 229 mg/L (3 rd day), 200 mg/L (5th day) and 118 mg/L (7th day). The cell free zoster which reflected the invasive ability was widened by tea polyphenols. The apoptosis percentage of CGTH W-3 cells incubated with tea polyphenols for 48 h was obviously increased, with 3.51% in control group, 12.61% with 100 mg/L tea polyphenols, 52.97% with 200 mg/L tea polyphenols and 70.79% with 400 mg/L tea polyphenols (all P
