Relationship between tissue factor and coagulation in acute vascular rejection of heart xenotransplantation
- VernacularTitle:异种心脏移植急性血管性排斥反应期组织因子与凝血的关系
- Author:
Xuan LU
;
Mingdi XIAO
;
Jinglai LI
- Publication Type:Journal Article
- Keywords:
Heart transplantation;
Graft rejection;
Thromboplastin
- From:
Chinese Journal of Organ Transplantation
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of tissue factor in the occurrence and development of co agulation in acute vascular rejection of heart xenotransplantation. Methods An animal model for acute vascular rejection of heart xenotransplantation was es tablished by using rat as recipient and guinea pig as donor. The xenografts were removed at 4, 8, 12, 16, and 24 h after the operation for fibrin sedimentation by immunohistochemical technique to evaluate coagulation. Meanwhile, the express ion of tissue factor mRNA was detected at different time points by RT-PCR. Hear ts of normal guinea pigs were used as controls. Results Immunohistochemical examination showed that coagu lation developed at the 8 h when interstitial fibrin deposition and fibrinous th rombus appeared and aggravated with time. High expression of guinea pig tissue factor mRNA appeared at 4 h after the transplantation and then steadily d eclined and totally terminated at the 16 h. The expression of rat tissue factor mRNA appeared 16 h post operation and then kept steadily enhanced. Conclusions The tissue factor acts as an important role in the acute vascular rejection of h eart xenotransplantation. The high expression of donor tissue factor mRNA might be related to the trigger of coagulation and the high expression of the receptor ’s tissue factor may be related to the further development of coagulation.
