Effect of calcitriol and aminoguanidine on acute renal allograft rejection in rats
- VernacularTitle:骨化三醇和氨基胍联合应用减轻大鼠肾移植急性排斥反应
- Author:
Yanzong ZHAO
;
Zhongjin YUE
- Publication Type:Journal Article
- Keywords:
Calcitriol;
Aminoguanidine;
Kidney transplantation;
Graft rejection
- From:
Chinese Journal of Organ Transplantation
2005;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the methods of induced tolerance in rats renal allotransplantation and provide a theoretical basis for clinical use. Methods Orthotopic renal transplantation from Wistar rats to SD rats was used as a model of study. The experimental rats were divided into the following groups: Group Ⅰ as isotransplantation control group, Ⅱ as acute rejection control group, respectively. Group Ⅲ, Ⅳ and Ⅴ were treated with aminoguanidine (AG), calcitriol and the combined use of calcitriol and AG, respectively. The survival time of recipients was observed, meanwhile, the donor specific mixed lymphocyte reaction (MLR) and concentrations of IFN-?, Ca 2+ and P 3+ in serum were measured. HE staining inspected all specimens of kidney grafts. Results The recipient survival time in the groups Ⅰ, Ⅱ, Ⅲ, Ⅳ and Ⅴ were ( 9.1 ? 1.9 ),( 5.3 ? 0.8 ),( 9.7 ? 2.1 ),( 8.6 ? 1.6 )and( 12.9 ? 3.4 )days, respectively. There was significant difference in recipient survival time between group Ⅴ and group Ⅱ, Ⅲ and Ⅳ (P
