Construction of recombinant nonstructural P_2C plasmid in coxsackie B4 virus
- VernacularTitle:柯萨奇B4病毒非结构蛋白P_2C重组质粒的构建
- Author:
Jiuchun GAO
;
Honglan HUANG
;
Fan LI
- Publication Type:Journal Article
- Keywords:
Coxsackie B4 virus(CVB4);
Type 1 diabetes mellitus;
Nonstructural protein P_2C;
Recombinant plasmid
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct the recombinant nonstructural P_2C plasmid in coxsackie B4 virus.Methods:Using the RT-PCR,we fishing the nonstructural protein P_2C cDNA fraction from Coxsackie B4 virus(CVB4) and cloning into the PUCm-T vector, then transfer it into E.coli to construct the recombinant plasmid.Results:Using the total RNA of Coxsackie B4 virus as model, we amplied 987 bp fraction and cloned into PUCm-T vector identifying with BamH Ⅰ and Hind Ⅲ double enzyme.Its products were consistent with nonstructural protein P_2C PCR productions.Conclusion:The recombinant nonstructural P_2C plasmid was constructed succesfully in coxsackie B4 virus.
