Construction of enterotoxigenic Escherichia coli heat-stable enterotoxin fusion protein with glutathione S-transferase and detection of antibody against heat-stable enterotoxin
- VernacularTitle:肠毒素大肠杆菌耐热肠毒素与谷胱甘肽巯基转移酶融合蛋白的制备和耐热肠毒素抗体测定
- Author:
Jiping ZHENG
;
Xiangxin LIU
;
Lingchun WANG
;
Peng WANG
;
Shuqin LI
;
Zhaoshan ZHANG
- Publication Type:Journal Article
- Keywords:
Heat-stable enterotoxin;
Glutathione S-transferase;
Fusion protein;
Antibody detection
- From:
Chinese Journal of Immunology
2000;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To detection antibody against heat-stable enterotoxin by fusion protein.Methods:Mutant heat-stable enterotoxin precursor gene was ligated in vector pGEX-4T-2 to inductively express as a fusion protein GST/proST_m with glutathione S-transferase(GST).To investigate the antigenic action,serum and fecal antibodies against heat-stable enterotoxin was detected with this fusion protein.Results:The fusion protein was a about 32 kD protein.All the samples contain the antibody against ST.Conclusion:Such strategy was a promising method to detect antibody against heat-stable enterotoxin.
