Microchimerism in recipients after vascularized allogratf bone transplantation
- VernacularTitle:吻合血管同种异体骨移植术后微嵌合现象的研究
- Author:
Yun XIE
;
Zhenguang CHEN
;
Shengxiang TAO
;
Xiaohui ZHENG
;
Yuhua YANG
- Publication Type:Journal Article
- Keywords:
Bone transplantation;
Transplantation,homologous;
Microchimerism;
Sex-determining factor Y;
Immune tolerance
- From:
Chinese Journal of Trauma
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the status of microchimerism in recipients and the relation between microchimerism and immunologic tolerance after vascularized allograft bone transplantation. Methods X-ray and histological examinations were performed on recipients after massive vascularized shaft of femur from female Japanese white rabbit donors was transplanted to male recipients. Microchimerism in different organs and tissues were checked postoperatively using a semi-quantitative polymerase chain reaction (PCR) with a Y-chromosome specific primer at different time. Results X-ray and histological examinations showed typical bone union in the experiment group but irregular new bone calluses surrounded the transplanted bones, with high density sequestrum in the control group.Semiquantitative PCR with a Y-chyomosome specific primer indicated that the incidence of microchimerism in organs and tissues in the experiment group was higher than that in the control group postoperatively and increased with time. Conclusions After vascularized allograft bone transplantation, organs and tissues show microchimerism that has a positive correlation with the histocompatibility of the transplanted bones.
