Experimental Study on PPAR? Ligands Regulating the Inflammatory Cytokines in Human Gallbladder Epithelial Cells
- VernacularTitle:PPAR?配体调控人胆囊上皮细胞炎性因子表达的实验研究
- Author:
Jiangwen LIU
;
Nansheng CHENG
;
Guangdong PAN
;
Xianze XIONG
;
Lan YAN
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator-activated receptor ? Ligand Gallbladder epithelial cell (Interleukin) Cholecystitis
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the regulatory ability of peroxisome proliferator-activated receptor ?(PPAR?) ligands to the inflammatory response in human gallbladder epithelial cells. Methods Culture human gallbladder epithelial cells and identify them . Cells were treated for 24 hours with 0, 10 ?mol/L, 20 ?mol/L, 30 ?mol/L, 50 ?mol/L and 100 ?mol/L of Ciglitazone during cellular growth peak(5th day), then stimulated them with hIL-1? 5 ng/ml for 2 hours and measured the concentration of IL-6、IL-8 and TNF-? in cellular supernatants by riadioimmunoassay. Results Contrasted with control group, the expression of IL-6 and IL-8 in each test group were inhibited ((P
