Effects of Tumor Necrosis Factor-? on Vascular Endothelial Growth Factor and Matrix Metalloproteinase-9 Expression in Hepatic Cancer Cell Line HepG2
- VernacularTitle:TNF-?对肝癌细胞VEGF和MMP-9表达的影响
- Author:
Junhua ZHU
;
Kaixiong TAO
;
Guobin WANG
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-? Liver cancer cell Vascular endothelial growth factor Matrix metal protein enzyme-9
- From:
Chinese Journal of Bases and Clinics in General Surgery
2003;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether tumor necrosis factor-? (TNF-?) enhance the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9(MMP-9) in hepatic cancer cell line HepG2 or not. Methods Cultured HepG2 cells were treated by TNF-? with various concentration and time. The morphological changes of HepG2 cells were studied microscopically and the proliferation of HepG2 were detected by methyl thiazolyl tetrazolium (MTT). The expression of VEGF and MMP-9 mRNA in cultured HepG2 were determined by relative quantitative reverse transcription polymerase chain reaction. The VEGF and MMP-9 protein level in supernatants and in cytoplasm were determined by enzyme-linked immunosorbent assay (ELISA) and by immunocytochemical staining, respectively.Results There was a little morphological changes in HepG2 with TNF-? treatment, but no change of cell proliferation in corresponding time. The expression of VEGF and MMP-9 mRNA was enhanced gradually with the TNF-? concentration increasing, the VEGF and MMP-9 protein level in supernatants and in cytoplasm was elevated gradually with the concentration increasing. There was a dependance on the concentration when the concentration of TNF-? was lower than or equal to 10~4 U/L. Furthermore, the effect of promotion was close to peak when the TNF-? concentration up to 10~4 U/L; but no time-effect pattern observed. Conclusion TNF-? can enhance the expression of VEGF and MMP-9 at the level of mRNA and protein in hepatic cancer cell line.
