The effect of kidney-replenishing herb on the Indoleamine 2,3-dioxygenase of decidual macrophages
- VernacularTitle:补肾中药对蜕膜巨噬细胞吲哚胺2,3-二氧化酶的影响
- Author:
Xuelian LI
;
Suiqi GUI
;
Haiyan WANG
- Publication Type:Journal Article
- Keywords:
Indoleamine 2,3-dioxygenase;
Decidual macrophages;
Kidney-replenishing herb
- From:
Chinese Journal of Immunology
2001;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of Kidney-replenishing herb on the indoleamine 2,3-dioxygenase(IDO) of decidual macrophages.Methods:RT-PCR was applied to analyze the IDO mRNA transcription of decidue;Western blot was applied to analyze the expression of IDO protein on decidual macrophages;ELISA was applied to determinate the IL-10 and IFN-? concentration in culture supernates of decidual macrophages and lymphocytes; high-performance liquid chromatography was applied to determinate the kynurenine and tryptophan concentration in cell culture supernates, the ratio of kyn/try was used to determinate IDO activity.Results:The decidual IDO mRNA transcription of normal pregnancy is higher than that of inevitable abortion; the inhibitor of IDO make the balance of Th cytokine depart from Th2; the serum made from herbal medicine improve the expression of IDO protein and IDO activity, and resume the balance of Th cytokine.Conclusion:Appropriate IDO mRNA transcription and IDO activity of decidue is necessary for normal pregnancy;Kidney-replenishing herb improve IDO activity to appropriate value.
